|CONTACTE-mail : email@example.com
tel : +609-6683103
fax : +609-6683105
|PUBLICATIONKheng Leong Ooi, Suh In Loh, Mei Lan Tan, Tengku Sifzizul Tengku Muhammad, Shaida Fariza Sulaiman (2015) Growth inhibition of human liver carcinoma HepG2 cells and α-glucosidase inhibitory activity of Murdannia bracteata (C.B. Clarke) Kuntze ex J.K. Morton extracts. J. Ethnopharm. 162, 55-60
Guat-Siew Chew, Stephen Myers, Alexander Chong Shu-Chien, Tengku Sifzizul Tengku Muhammad (2014) Interleukin-6 inhibition of peroxisome proliferator-activated receptor alpha expression is mediated by JAK2- and PI3K-induced STAT1/3 in HepG2 hepatocyte cells. Mol. Cell. Biochem. 388, 25-37
Kheng Leong Ooi, Tengku Sifzizul Tengku Muhammad, Lee Yein Lam and Shaida Fariza Sulaiman (2014) Cytotoxic and Apoptotic Effects of Ethyl Acetate Extract of Elephantopus mollis Kunth. in Human Liver Carcinoma HepG2 Cells Through Caspase-3 Activation. Integr. Cancer. Ther. 13, NP1-NP9
Kheng Leong Ooi, Tengku Sifzizul Tengku Muhammad, Shaida Fariza Sulaiman (2013) Physalin F from Physalis minima L. triggers apoptosis-based cytotoxic mechanism in T-47D cells through the activation caspase-3 and c-myc-dependent pathways. J.Ethnopharm. 150, 382-388
Guat-Siew Chew, Stephen Myers, Kheng Leong Ooi, Alexander Chong Shu-Chien, Tengku Sifzizul Tengku Muhammad (2013) Interleukin-6 Induces the Down Regulation of Human Peroxisome Proliferator Activated Receptor Alpha via the MAPK-induced STAT Pathway in Human Hepatocytes. J Biochem. Pharmacol. Res. 1, 204-211
Lim, W.S., Ng, D.L., Kor, S.B., Wong, H.K., Tengku-Muhammad, T.S., Choo, Q.C., Chew, C.H. (2013) Tumour necrosis factor alpha down-regulates the expression of peroxisome proliferator activated receptor alpha (PPARα) in human hepatocarcinoma HepG2 cells by activation of NF-κB pathway. Cytokine 61, 266-274
Elucidation of the role of peroxisome proliferator activated receptor alpha (PPARα) in acute phase response
Identification of small molecules as therapeutic agents for atherosclerosis and diabetes using target-based high-throughput screening platforms
Cardiovascular disease (CVD) such as heart attacks still remains the leading cause of death worldwide. The underlying cause of CVD is atherosclerosis. One of the major risk factors of atherosclerosis is hypercholesterolaemia. High-density lipoprotein (HDL) is an important molecule that involves in reverse cholesterol transport by delivering the cholesterol to the liver via a receptor present on the surface of liver cells known as Scavenger Receptor-Type B1 (SR-B1), a PPAR target gene. Our lab has successfully developed a high-throughput platform utilisng SR-B1 as the target for the screening of small molecules (natural and synthetic compounds) with a potent activity in reducing blood cholesterol levels.
Diabetes mellitus is a leading metabolic disorder that leads to high blood glucose levels due to resistance or insufficient production of insulin. It was widely reported that the incidence of atherosclerosis is accelerated in patients with type II diabetes. Curently, one of the widely use drugs for type 2 diabetes is Thiazolidinediones (TZD). These molecules act by binding and activating PPARg as its ligands. Our lab has established a cell-based high throughput screening platform by utilizing a chimaeric plasmid harbouring PPARg binding sites (peroxisome proliferator responsive elements, PPRE) linked to a reporter system, luciferase. Small molecules provided by our collaborators will be subjected to the developed screening platform to identifiy the compounds with potent activity as PPARg ligand.
In addition, our lab is also involved in the identification of small molecules that exhibit the cytotoxicity activity in breast, cervical and liver cancer cells.
The mechanisms of action of the potent molecules in the regulating the cellular and molecular events in atherosclerosis, diabetes and cancer are also elucidated.
|SCOPUS AUTHOR ID : 6507176556|
| Kheng Leong Ooi, Tengku Sifzizul Tengku Muhammad, Mei Lan Tan and Shaida Fariza Sulaiman (2011) Cytotoxic, Apoptotic and Anti-a-glucosidase Activities of 3,4-di-O-caffeoyl Quinic Acid, an Antioxidant Isolated from the Polyphenolic-rich Extract of Elephantopus mollis Kunth. J. Ethnopharm. 135, 685-695Belal O. Al-Najjar, Habibah A. Wahab, Tengku Sifzizul Tengku Muhammad, Alexander Chong Shu-Chien, Nur Adelina Ahmad Noruddin and Mutasem O. Taha (2011) Discovery of New Nanomolar Peroxisome Proliferator-Activated Receptor-g Activators via Elaborate Ligand-based Modeling. Eur. J. Med. Chem. 46, 2513-2529Ooi KL, Tengku Muhammad TS, Lim CH, Sulaiman SF (2010) Apoptotic Effects of Physalis minima L. Chloroform Extract in Human Breast Carcinoma T-47D Cells Mediated by c-myc, p53-, and caspase-3 -Dependent Pathways. Integr. Cancer Ther. 9, 73-83
Ooi KL, Tengku Muhammad TS, Sulaiman SF (2010) Growth arrest and induction of apoptotic and non-apoptotic programmed cell death by, Physalis minima L. chloroform extract in human ovarian carcinoma Caov-3 cells. J. Ethnopharm. 128, 92-99
Choy-Hoong Chew, Guat-Siew Chew, Nazalan Najimudin and Tengku Sifzizul Tengku-Muhammad (2007) Interleukin-6 inhibits human peroxisome proliferator activated receptor alpha gene expression via CCAAT/enhancer-binding proteins in hepatocytes. Int. J. Biochem. Cell Biol. 39, 1975-1986