Tentative Programmes
Advanced Training in DNA Barcoding and Introduction to Environmental DNA (eDNA) Metabarcoding for Aquatic Ecosystem Conservation
DAY 1 (TUESDAY)
TIME
TOPIC/ ACTIVITY
SPEAKERS
ICAMB Seminar Room
8.00 am
Registration
Admin staff
8.45 am
Introduction – DNA Barcoding and eDNA metabarcoding, case study of Malaysia
Prof. Dr. Siti Azizah Mohd Nor (UMT)
9.45 am
Tea Break
All
ICAMB Preparation Lab
10.00 am
Topic 1: Biodiversity sampling and sample processing
Lecture and hands-on training
Dr. Tun Nurul Aimi Mat Jaafar (UMT)
12.30 am
Lunch
All Attendees
ICAMB Proteomic Lab (Level 2)
2.00 pm
Topic 2: Molecular work (laboratory procedures)
Lecture and hands-on training on DNA extraction, PCR, gel electrophoresis, DNA sequencing
Assoc. Prof. Dr. Tan Min Pau (UMT)
3.30 pm
Tea Break
All Attendees
3.45 pm
Topic 2: Molecular work (laboratory procedures)
- Continuation
Dr. Norfatimah Mohd Yunus (UiTM)
4.30 pm
End
DAY 2 (WEDNESDAY)
TIME
TOPIC/ ACTIVITY
SPEAKERS
ICAMB Seminar Room
9.00 am
Topic 3: DNA barcoding and phylogenetic analyses
Lecture and hands-on training of sequence analysis
Sequence trimming, MEGAX
Basic tree building – NJ, ML
Dr. Sebastien Lavoue (USM)
10.30 am
Tea Break
All Attendees
10.45 am
Topic 3: DNA barcoding and phylogenetic analyses – Continuation
Lecture and hands-on training of sequence analysis
BOLD – sequence submission, analysis – RESL (BIN), barcode gap
Dr. Jamsari Amirul Firdaus Jamaludin (USM)
12.30 pm
Lunch
All Attendees
2.00 pm
Topic 3: DNA barcoding and phylogenetic analyses – Continuation
Lecture and hands-on training of sequence analysis
Introduction to species delimitation analysis – GMYC, bPTP etc
Ms. Noorul Azliana Jamaluddin (IPP, Jabatan Perikanan Malaysia)
Ms. Siti Zafirah Ghazali (UMT)
4.30 pm
Tea Break
End
All Attendees
DAY 3 (THURSDAY)
TIME
TOPIC/ ACTIVITY
SPEAKERS
ICAMB Seminar Room
9.00 am
Topic 4: Introduction to eDNA metabarcoding for biodiversity assessment
Case study of Merbok Estuary and selected lakes in Peninsular Malaysia
Dr. Danial Hariz Zainal Abidin (USM)
Ms. Norli Fauzani Abu Hassan Alshari (UMT)
10.30 am
Tea Break
All Attendees
10.45 am
Topic 4: Introduction to eDNA metabarcoding for biodiversity assessment – Continuation
Environmental sample collection, eDNA library preparation, and next generation sequencing
*quick hands-on on water filtration
Dr. Sebastien Lavoue (USM)
Ms Masazurah Ab Rahim (IPP, Jabatan Perikanan Malaysia )
Ms Nur Ilham Syahadah Mohd Yusoff (UMT)
Ms. Noorhani Syahida Kasim (UMT)
12.30 pm
Lunch
All Attendees
2.00 pm
Topic 4: Full Length Amplicon Sequencing and its Application
Overview of NGS data analysis and basic demo on data analysis using free online tools – mBRAVE platform
Dr. Danial Hariz Zainal Abidin (USM)
Ms. Norli Fauzani Abu Hassan Alshari (UMT)
3.00 pm
Topic 5: Full Length Amplicon Sequencing and its Applications
Dr. Kamal Ghazali (Apical Scientific)
Mr. Liew Yee Sen (Apical Scientific)
3.45 pm
Topic 6. Exploring opportunities for future collaborations
All Attendees
5.00 pm
5.30 pm
Closing Remarks and Tea
End
Accoc. Prof. Dr. Tan Min Pau